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Research: Shared Services

Proteomics Services

Equipment:

• One and Two Dimensional Gel Electrophoresis: We offer state-of-the-art equipment from Proteome Systems Technologies, which is fully compatible with all the rest of the high-throughput platform. This includes a Shimadzu Xcise robot, which excises, digests, cleans up the proteins from the gel, deposits them on an x,y sample platform and adds the matrix as required for automated MALDI analysis.



• Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometer: The Axima MALDI-TOF mass spectrometer from Kratos/Shimadzu provides a combination of high-mass accuracy, high resolution and high sensitivity. The MS includes high-throughput sample handling using a 384-spot microtiter sample format MALDI target on an x,y stage, mass correlated acceleration and a curved field reflectron. Bioinformatics will provide protein identification based on proteolytic peptide maps.



• A Finnigan ProteomeX Ion Trap Spectrometer interfaced via Electrospray, and offering tandem mass spectrometry capability and TurboSEQUEST software for protein bioinformatics. This is a high-throughput system with reverse phase columns for rapid MS/MS analysis of peptides and, thereby, identification of proteins in mixtures.



• A Finnigan LTQ-Orbitrap offers a tremendous advantage because of its m/z accurate measurements, down to 10-2 ppm. This accuracy becomes of great advantage when tryingA to detect much less abundant peptides and proteins as well as when trying to determine site specific post translational modifications.



• An AKTA FPLC Protein Purification System from Amersham is a biocompatible, high-performance liquid chromatography system especially designed and proven worldwide for fast and easy purification of proteins on a wide variety of columns.



• Compugen Z3 Software for analysis of gel arrays. This is state-of-the-art software to facilitate digitization, annotation and quantitative comparisons of gel arrays.

Services and Charges:

*For trained and authorized users only
**Multiple sample rates to be discussed with Core personnel
***Requires investigators to perform their own sample digests

All Private and Rush Orders are Double the Price.

Note: The Proteomics Shared Service will, upon request, provide a description of our services for use in your grant applications under "Resources."

 

Faculty Advisory Committee:

Alan Tomkinson, Professor, Radiation Oncology
Peter Swaan, Assistant Professor, School of Pharmacy
Dan Fabris, Associate Professor, Department of Chemistry, UMBC
Edward Sausville, Professor, Department of Medicine
Nicholas Ambulos, Associate Professor, Department of Microbiology
Dudley Strickland, Professor, Department of Immunology
David Webber, Professor, Department of Biochemistry
John Sauk, Professor, School of Dentistry
Austin Yang, Associate Professor, Department of Neurobiology
Peter Gutierrez, Professor, Department of Biochemistry
William Rodgers, Associate Professor, Department of Pathology

Partial List of Users:

Brodie, Angela; Cullen, Kevin; Fasano, Alesio; Tomkinson, Alan; Fenselau, Catherine; Hamburger, Anne; Kwong, King; Lu-Chang, A-Lien; Serrero Ginette; Strome, Scott; Burger, Angelika; Gnatt, Averell; Bernstein, Steven; Black, Linsay; Bloch, Robert; Dickson, R; Hampton, Brian; Kwon, Moo; Medved, A; Qin, Calvin; Lee, James

This page was last updated on: January 21, 2010.

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