A Part of the University of Maryland Medical Center

Connect with UMGCC
Facebook Twitter YouTube Blog iPhone
Email PageEmail page Print PagePrint page

Research Programs

Costs And Basic Guidelines For Injection Services

Pronuclear Microinjection of a DNA construct:

(injection into the pronucleus to create transgenic founder mice)

Strain Cost Parameters* Experimental Goals **
B6C3 F1 hybrid $2000 200 eggs injected 3 founders or 20 live pups
FVB albino $2000 200 eggs injected 3 founders or 20 live pups
C57Bl6 $2750 200 eggs injected 2 founders or 15 live pups

*the number of eggs injected includes ~50 used for an initial test of DNA quality
**re-injection of 100 eggs will be done at no additional charge under certain conditions

Other strains can be used for microinjection under the following conditions. The investigator will pay for the purchase and housing of 10 stud male and 10 donor female mice. The facility will attempt one day of microinjection at a charge of $1000. The injections will continue only if 100 injectable eggs are obtained. Total charges will not exceed $2000 plus costs associated with purchase and housing of animals.

back to top

Blastocyst Microinjection of Embryonic Stem Cell Clones:

(generation of chimeras, 2-4 clones injected)

Strain Cost Parameters Experimental Goals*
C57Bl6 $3000 80 blastocysts 4 chimeras or 30 live pups injected

*re-injection of 30 blasts will be done at no additional charge under certain conditions

Other strains can be used for microinjection under the following conditions. The investigator will pay for the purchase and housing of 10 stud male and 10 donor female mice. The facility will attempt one day of microinjection at a charge of $1500. The injections will continue only if 40 injectable blastocysts are obtained. Total charges will not exceed $3000 plus costs associated with purchase and housing of animals.

back to top

This page was last updated on: January 19, 2007.