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Research Programs

High Throughput Screening (HTS) Shared Service: Services

There are three main components to the successful operation of the HTS SS: instrumentation, compound libraries, and technical staff to consult on optimizing assays. The HTS SS operates two types of instruments, automated liquid handling workstations to enable high throughput processing, and microplate readers equipped with a variety of detection mechanisms. The HTS SS provides the investigators access to the equipment needed to perform HTS, compound libraries for screening, consulting and training, and can perform all aspects of a screening project if necessary.

Equipment Use

The HTS SS allows investigators to operate the equipment themselves, providing training and oversight only, for set hourly rates. The equipment includes the automated laboratory workstations and the microplate readers:

Compound Libraries

Portions of compound libraries can be purchased for use by the investigator as needed for their screens either performed in the HTS SS or in their own lab. This provides the investigator access to the full diversity of the library without having to incur the cost of buying the entire library. There is a cost to the aliquot and reformatting the library that is done by the HTS SS staff to assure the library stocks are maintained properly. The current libraries available our as follows:

Technical Expertise and Customized Services

The HTS SS can be involved in all or just partial aspects of the screen development, transferring it to an automated platform, to performing all aspects of the day to day screening such as maintain cell lines for cellular assays, running sample, and data mining and organization. These services are quoted out on an individual basis due to each assay being very individual using different assay plates, media, reagents, etc., and requiring varying time and equipment, therefore, the final costs cannot be fully determined until this final version of the assay is set. The general scope of the assays can also vary from and investigator using the robotics to perform a simple task that may take only a day, to long term projects that span over several years.

NMR Based Screening

In collaboration with the UMB NMR Center, it is possible to use the Bruker 800 MHz NMR with the B-ACS 60 Automatic Sample Changer for automated NMR spectra collection on proteins and other molecules. This is a very unique instrument in that typically autoloaders are not installed on high field NMRs that will allow spectra of of proteins to be determined rapidly and be used to screen for protein-compound interaction. Change in the NMR spectra of molecules occur when they bind ligands allowing the interaction of molecules to be determined over a wide range of affinities making it an ideal instrument for screening fragment libraries that may only bind with micro to millimolar affinities.

This page was last updated on: March 3, 2010.