Click here for information on Users of the HTS Shared Service.
David Weber, Ph.D., Director. Dr. David Weber, Professor in the Department of Biochemistry and Molecular Biology, is the NMR Center Director at UMB and has extensive experience in high-throughput screening as well in operating a shared service facility. He supervises the manager of the HTS SS and administers the policies of the HTS SS with advice from the faculty advisory committee and Dr. Nick Ambulos, the director of Shared Services. In addition, Dr. Weber has volunteered the use of several pieces of equipment from his lab including a Beckman Coulter Biomek NXp Laboratory Workstation and a BMG POLARstar microplate reader as well as the smaller Spectrum Collection compound library. Since Dr. Weber is also the director of the UMB NMR center, it is routine for the HTS Center to work closely with the NMR Center on some projects. The UMB NMR center has an 800 MHz NMR spectrometer that is equipped with a cryogenic probe and an auto-sampler loader, which allows for directly monitoring the binding of compounds to proteins using structure-based screening assays via state-of-the-art NMR spectroscopy techniques.
Paul Wilder, Ph.D., Manager. The everyday operations of the HTS SS are performed by the manager, Dr. Paul Wilder, who is directly responsible for maintaining the equipment in the HTS including the automated liquid handling equipment and fluorescent plate readers. Dr. Wilder oversees the small compound libraries to assure the highest quality of the compounds are available, preventing contamination, and allowing tracking the compounds and dilutions to allow the user to reorder the correct compounds if needed. The screens may be directly performed by Dr. Wilder, but he will alternatively train users on the equipment and assist in moving the researchers' assay from the bench-top to a high throughput format. Dr. Wilder has experience in both academia and industry in the development of both cellular and in vitro assays. Previously, in his industrial experience at Alpha-Beta Technology, Inc., he developed and performed assays to assure the purity and efficacy of biologic therapeutics. He gained even more experience in cellular assay development at ViaCell, Inc. giving me a broad knowledge base including ELISA, FACS, cell separation/purification, and stem cell culture. In his Ph.D. training, he performed biochemical and biophysical studies using a wide range of techniques including protein structural studies, cellular and molecular biology including NMR, fluorescence spectrophotometry, ITC, and protein purification. During his postdoctoral training, he focused on structural based drug design and HTS providing the training using laboratory automation systems that eventually led to a position as the manager of the HTS SS. In addition, he has a great deal of experience training and managing lab personnel in both academia and in industry where he headed four full-time employees and several part-time employees and interns. In total, he has the experience and skills necessary to run a successful HTS SS for the UMGCC.