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Flow Cytometry: Instrumentation

Aria

13 colors 4-way sorter
$60.00 for 30 minutes of sorting, sterile set up fee, (other charges)
http://cf.umaryland.edu/freezer/promo_fcs.cfm

The BD FACSAria flow cytometer is the first fixed-alignment bench top high-speed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at a time. The BD FACSAria system consists of three major components: a fluidics cart, a bench top flow cytometer, and a workstation. Nearly all instrument functions are operated from within BD FACSDiva software.

BD FACSAria User Guide.

FACScan

The FACScan is capable of 3 color detection off a 488nm laser. It is great for DNA cell cycle or screening, Apoptosis, GFP, and other simple assays.

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired

LSRII

14 color analyzer

The LSR II is equipped with 5 lasers; 488nm blue laser, 561 nm yellow/green laser, 640nm red diode laser, 405nm violet laser, and 350nm UV laser. Capable of simultaneous detection of 14 fluorochromes and 16 parameter data acquisition. It is a digital instrument using FACSDiva software.

BD LSRII User Guide

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired

http://cf.umaryland.edu/freezer/promo_fcs.cfm

CANTO

The BD FACSCanto system sets a new standard for performance in flow cytometry. With its fixed-optics design and digital electronics, the BD FACSCanto flow cytometer allows multicolor analysis of up to six fluorescent markers and two scatter parameters at a time.

BD CANTO User Guide

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired

http://cf.umaryland.edu/freezer/promo_fcs.cfm

This page was last updated on: January 22, 2013.

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