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The UMGCC Flow Cytometry facility was established as a core in 1998 when Dr. Dean L. Mann became director. Dr. Mann is Professor of Pathology and Director of the Division of Immunogenetics, and is recognized internationally for his work in this field. He was Co-Director of the first flow cytometry facility at the National Cancer Institute, NIH.
In 1998, Dr. Mann, with the assistance of other UMGCC members, successfully applied for a grant through the Shared Resources for Scientists Outside NCI Cancer Center’s Program. This grant, together with other outside financial support from the cancer center, as well as a gift from Searle-Pharmacia, provided funds to establish the current flow cytometry facility.
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The instrumentation we now have for acquisition and analysis consists of an upgraded 3-color FACScan, with a fixed 488nm laser and a 6-color BD-LSR, with three lasers (argon 488nm, helium-neon 633nm and helium-cadmium 325 for ultraviolet excitation).
For cell sorters, we have a FACSVantage SE, with two lasers (krypton-argon mixed gas laser 488nm/351nm and a helium-neon 633nm). This sorter has six detectors for use in complex analysis and cell sorting. The second sorter, FACSVantage DiVa, is one of Becton Dickinson’s most advanced flow cytometers available. It is equipped with three lasers (argon 488nm, krypton-ion laser 407nm and helium-neon 633nm), with advanced optics and new generation of software, this instrument provides enormous flexibility in terms of applications as well as unprecedented sensitivity.
In addition, a MoFlo High-Performance Cell Sorter is being considered for a third sorter. This instrument has the largest selection of footprints and laser configurations; the most number of unique productivity tools; the highest sort yields with full viability and function, and unparalleled instrument performance.
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