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Research: Shared Services

Flow Cytometry: Instrumentation

Aria

Photos of Flow Cytometry: Aria

9 parameters, 7 color 4-way sorter

$60.00 for 30 minutes of sorting, sterile set up fee, (other charges)http://cf.umaryland.edu/freezer/promo_fcs.cfm

The BD FACSAria flow cytometer is the first fixed-alignment bench top high-speed cell sorter. The instrument can be operated at varied pressures and can acquire up to 70,000 events per second. With its fixed-optics design and digital electronics, the BD FACSAria flow cytometer enables multicolor analysis with two high power lasers: 488nm blue sapphire and 633 red H3-N3 laser. The BD FACSAria system consists of three major components: a fluidics cart, a bench top flow cytometer, and a workstation. Nearly all instrument functions are operated from within BD FACSDiva software.

BD FACS Aria Uer Guide.

Aria II

Photos of Flow Cytometry: Aria II

The BD FACS Aria II is a high speed cell sorter for measuring and sorting fluorescent labeled cells. It is equipped with 3 lasers; 488nm blue laser, 640nm red diode laser and 405nm violet laser. This sorter is capable of simultaneous detection of 9 flurochromes, and 11 parameter data acquisitions. It is a digital instrument using FACSDiva software. The BD FACSAriaII system consists of three major components: a fluidcs cart, a bench top flow cytometer and a workstation.


FACScan

Photos of Flow Cytometry: FACScan

The FACScan is capable of 3 color detection off a 488nm laser. It is great for DNA cell cycle or screening, Apoptosis, GFP, and other simple assays.

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired


LSRII

Photos of Flow Cytometry: LSRII

14 color analyzer

The LSR II is equipped with 5 lasers; 488nm blue laser, 561 nm yellow/green laser, 640nm red diode laser, 405nm violet laser, and 350nm UV laser. Capable of simultaneous detection of 9 fluorochromes and 11 parameter data acquisition. It is a digital instrument using FACSDiva software.

BD LSRII User Guide

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired

HTS: The LSRII has a high throughput sampling options. This system can sample 96 to 384 well plates. Analysis of all 96 wells from a 96 well plate can be done in as little as 15 minutes (actual time depends on users selectable parameters and samples). Carryover between samples is less than 1%.

$50.00 for HTS set up and tare down fee, plus $12.50/every 15 minutes on instrument.

http://cf.umaryland.edu/freezer/promo_fcs.cfm


CANTO

Photos of Flow Cytometry: CANTO

The BD FACSCanto system sets a new standard for performance in flow cytometry. With its fixed-optics design and digital electronics, the BD FACSCanto flow cytometer allows multicolor analysis of up to six fluorescent markers and two scatter parameters at a time.

BD CANTO User Guide

$25.00/15 minutes operator acquired
$12.50/15 minutes user acquired

http://cf.umaryland.edu/freezer/promo_fcs.cfm


This page was last updated on: February 25, 2014.